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Applied and Environmental Microbiology, November 2008, p. 6797-6802, Vol. 74, No. 21
0099-2240/08/$08.00+0     doi:10.1128/AEM.00518-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Joint Immobilization of Plant Growth-Promoting Bacteria and Green Microalgae in Alginate Beads as an Experimental Model for Studying Plant-Bacterium Interactions{triangledown}

Luz E. de-Bashan and Yoav Bashan*

Department of Soil, Water and Environmental Science, The University of Arizona, Tucson, Arizona 85721, and Environmental Microbiology Group, Northwestern Center for Biological Research (CIBNOR), Mar Bermejo 195, Col. Playa Palo de Santa Rita, La Paz, BCS 23090, Mexico

Received 3 March 2008/ Accepted 4 September 2008

A simple, quantitative experimental model, offering a convenient and basic approach to studies of plant-bacterium interactions, is proposed. This involves immobilizing a unicellular, freshwater microalga, a Chlorella species, serving as the plant, with a plant growth-promoting bacterium, an Azospirillum species, in small alginate beads to allow close interaction and to avoid external interference from bacterial contaminants.

* Corresponding author. Mailing address: Environmental Microbiology Group, Northwestern Center for Biological Research (CIBNOR), Mar Bermejo 195, Col. Playa Palo de Santa Rita, La Paz, BCS 23090, Mexico. Phone: 52-612-123-8484. Fax: 52-612-125-4710. E-mail: bashan{at}cals.arizona.edu

{triangledown} Published ahead of print on 12 September 2008.

Applied and Environmental Microbiology, November 2008, p. 6797-6802, Vol. 74, No. 21
0099-2240/08/$08.00+0     doi:10.1128/AEM.00518-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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