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Applied and Environmental Microbiology, November 2008, p. 6803-6805, Vol. 74, No. 21
0099-2240/08/$08.00+0     doi:10.1128/AEM.00873-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Metagenomic Screening for Bleomycin Resistance Genes{triangledown} ,{dagger}

Toshio Mori,1 Shiori Mizuta,1 Hikaru Suenaga,1 and Kentaro Miyazaki1,2*

Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan,1 Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan2

Received 16 April 2008/ Accepted 5 September 2008

A metagenomic library of activated sludge was screened for bleomycin resistance genes. Two genes were identified that differed greatly from each other, from the genes of bleomycin-producing actinomycetes, and from those of clinical isolates. Therefore, the nonclinical environment is a rich reservoir of new resistance elements, and metagenomics can be used to sample the resistome rapidly.

* Corresponding author. Mailing address: Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. Phone: 81-29-861-6060. Fax: 81-29-861-6413. E-mail: miyazaki-kentaro{at}aist.go.jp

{triangledown} Published ahead of print on 12 September 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, November 2008, p. 6803-6805, Vol. 74, No. 21
0099-2240/08/$08.00+0     doi:10.1128/AEM.00873-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Martinez, J. L. (2009). The role of natural environments in the evolution of resistance traits in pathogenic bacteria. Proc R Soc B 276: 2521-2530 [Abstract] [Full Text]  


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