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Applied and Environmental Microbiology, November 2008, p. 6806-6807, Vol. 74, No. 21
0099-2240/08/$08.00+0     doi:10.1128/AEM.01047-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Simple and Safe Method for Simultaneous Isolation of Microbial RNA and DNA from Problematic Populations{triangledown}

Simon McIlroy, Kate Porter, Robert J. Seviour, and Daniel Tillett*

Biotechnology Research Centre, La Trobe University, Bendigo, Victoria, Australia

Received 9 May 2008/ Accepted 29 August 2008

We describe a novel, rapid, and safe method for extracting RNA and DNA from refractory microbes, which avoids the use of phenol or chloroform. It has been used successfully to isolate high-quality nucleic acids from pure cultures and environmental populations known to resist widely used extraction protocols.

* Corresponding author. Mailing address: Biotechnology Research Centre, La Trobe University, P.O. Box 199, Bendigo, VIC 3552, Australia. Phone: 61 3 5444 7361. Fax: 61 3 5444 7476. E-mail: d.tillett{at}latrobe.edu.au

{triangledown} Published ahead of print on 12 September 2008.

Applied and Environmental Microbiology, November 2008, p. 6806-6807, Vol. 74, No. 21
0099-2240/08/$08.00+0     doi:10.1128/AEM.01047-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Speirs, L., Nittami, T., McIlroy, S., Schroeder, S., Seviour, R. J. (2009). Filamentous Bacterium Eikelboom Type 0092 in Activated Sludge Plants in Australia Is a Member of the Phylum Chloroflexi. Appl. Environ. Microbiol. 75: 2446-2452 [Abstract] [Full Text]  


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