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Applied and Environmental Microbiology, November 2008, p. 6808-6810, Vol. 74, No. 21
0099-2240/08/$08.00+0     doi:10.1128/AEM.00787-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Reconstitution of Iron Oxidase from Sulfur-Grown Acidithiobacillus ferrooxidans{triangledown} ,{dagger}

Taher M. Taha,1* Tadayoshi Kanao,1 Fumiaki Takeuchi,2 and Tsuyoshi Sugio1

Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, 1-1-3 Tsushima-naka, Okayama 700-8530, Japan,1 Environmental Management Center, Okayama University, 1-1-3 Tsushima-naka, Okayama 700-8530, Japan2

Received 7 April 2008/ Accepted 30 August 2008

The iron oxidation system from sulfur-grown Acidithiobacillus ferrooxidans ATCC 23270 cells was reconstituted in vitro. Purified rusticyanin, cytochrome c, and aa3-type cytochrome oxidase were essential for reconstitution. The iron-oxidizing activity of the reconstituted system was 3.3-fold higher than that of the cell extract from which these components were purified.

* Corresponding author. Mailing address: Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, 1-1-3 Tsushima-naka, Okayama 700-8530, Japan. Phone and fax: 81-86-251-8306. E-mail: tahery1{at}yahoo.com

{triangledown} Published ahead of print on 12 September 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, November 2008, p. 6808-6810, Vol. 74, No. 21
0099-2240/08/$08.00+0     doi:10.1128/AEM.00787-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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