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 Previous Article

Applied and Environmental Microbiology, November 2008, p. 6820-6823, Vol. 74, No. 21
0099-2240/08/$08.00+0     doi:10.1128/AEM.00388-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Type II Hydride Transferases from Different Microorganisms Yield Nitrite and Diarylamines from Polynitroaromatic Compounds{triangledown} ,{dagger}

Pieter van Dillewijn, Rolf-Michael Wittich, Antonio Caballero,{ddagger} and Juan-Luis Ramos*

Departamento de Protección Ambiental, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Apdo. Correos 419, E-18008 Granada, Spain

Received 15 February 2008/ Accepted 4 September 2008

Homogenous preparations of XenB of Pseudomonas putida, pentaerythritol tetranitrate reductase of Enterobacter cloacae, and N-ethylmaleimide reductase of Escherichia coli, all type II hydride transferases of the Old Yellow Enzyme family of flavoproteins, are shown to reduce the polynitroaromatic compound 2,4,6-trinitrotoluene (TNT). The reduction of this compound yields hydroxylaminodinitrotoluenes and Meisenheimer dihydride complexes, which, upon condensation, yield stoichiometric amounts of nitrite and diarylamines, implying that type II hydride transferases are responsible for TNT denitration, a process with important environmental implications for TNT remediation.

* Corresponding author. Mailing address: EEZ-CSIC, C/Profesor Albareda 1, 18008 Granada, Spain. Phone: 34 958 181608. Fax: 34 958 135740. E-mail: jlramos{at}eez.csic.es

{triangledown} Published ahead of print on 12 September 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} Present address: Göteborg University, Cmb. Microbiologi Box 462, 405 30 Göteborg, Sweden.

Applied and Environmental Microbiology, November 2008, p. 6820-6823, Vol. 74, No. 21
0099-2240/08/$08.00+0     doi:10.1128/AEM.00388-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Roca, A., Rodriguez-Herva, J. J., Ramos, J. L. (2009). Redundancy of Enzymes for Formaldehyde Detoxification in Pseudomonas putida. J. Bacteriol. 191: 3367-3374 [Abstract] [Full Text]  
  • van Dillewijn, P., Wittich, R.-M., Caballero, A., Ramos, J.-L. (2008). Subfunctionality of Hydride Transferases of the Old Yellow Enzyme Family of Flavoproteins of Pseudomonas putida. Appl. Environ. Microbiol. 74: 6703-6708 [Abstract] [Full Text]  


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