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Applied and Environmental Microbiology, November 2008, p. 7070-7072, Vol. 74, No. 22
0099-2240/08/$08.00+0     doi:10.1128/AEM.00681-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Flavobacterium sp. Strain 4221 and Pedobacter sp. Strain 4236 β-1,3-Glucanases That Are Active at Low Temperatures {triangledown} ,{dagger}

Mikkel A. Rasmussen,1 Søren M. Madsen,1 Peter Stougaard,2 and Mads G. Johnsen1*

Bioneer A/S, Kogle Allé 2, DK-2970 Hørsholm, Denmark,1 Department of Ecology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark2

Received 24 March 2008/ Accepted 12 September 2008

Secretion of β-1,3-glucanases by the arctic bacterial isolates 4221 and 4236, related to the genera Flavobacterium and Pedobacter, was discovered. Escherichia coli and Lactococcus lactis expression of β-1,3-glucanases Glc4221-1 and Glc4236-1 from the respective isolates was achieved. The enzymes hydrolyzed fungal cell walls and retained activity at low temperatures.

* Corresponding author. Mailing address: Bioneer A/S, Kogle Allé 2, DK-2970 Hørsholm, Denmark. Phone: 45 4516 0444. Fax: 45 4516 0455. E-mail: mgj{at}bioneer.dk

{triangledown} Published ahead of print on 19 September 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, November 2008, p. 7070-7072, Vol. 74, No. 22
0099-2240/08/$08.00+0     doi:10.1128/AEM.00681-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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