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Applied and Environmental Microbiology, December 2008, p. 7252-7257, Vol. 74, No. 23
0099-2240/08/$08.00+0     doi:10.1128/AEM.01997-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Differential Expression in Phanerochaete chrysosporium of Membrane-Associated Proteins Relevant to Lignin Degradation {triangledown} ,{dagger}

Semarjit Shary,1,2 Alexander N. Kapich,1,2 Ellen A. Panisko,3 Jon K. Magnuson,3 Daniel Cullen,1,2 and Kenneth E. Hammel1,2*

Institute for Microbial and Biochemical Technology, USDA Forest Products Laboratory, Madison, Wisconsin 53726,1 Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706,2 Chemical and Biological Processes Development Group, Pacific Northwest National Laboratory, Richland, Washington 993523

Received 27 August 2008/ Accepted 2 October 2008

Fungal lignin-degrading systems likely include membrane-associated proteins that participate in diverse processes such as uptake and oxidation of lignin fragments, production of ligninolytic secondary metabolites, and defense of the mycelium against ligninolytic oxidants. Little is known about the nature or regulation of these membrane-associated components. We grew the white rot basidiomycete Phanerochaete chrysosporium on cellulose or glucose as the carbon source and monitored the mineralization of a 14C-labeled synthetic lignin by these cultures to assess their ligninolytic competence. The results showed that the cellulose-grown cultures were ligninolytic, whereas the glucose-grown ones were not. We isolated microsomal membrane fractions from both types of culture and analyzed tryptic digests of their proteins by shotgun liquid chromatography-tandem mass spectrometry. Comparison of the results against the predicted P. chrysosporium proteome showed that a catalase (Joint Genome Institute P. chrysosporium protein identification number [I.D.] 124398), an alcohol oxidase (126879), two transporters (137220 and 132234), and two cytochrome P450s (5011 and 8912) were upregulated under ligninolytic conditions. Quantitative reverse transcription-PCR assays showed that RNA transcripts encoding all of these proteins were also more abundant in ligninolytic cultures. Catalase 124398, alcohol oxidase 126879, and transporter 137220 were found in a proteomic analysis of partially purified plasma membranes from ligninolytic P. chrysosporium and are therefore most likely associated with the outer envelope of the fungus.

* Corresponding author. Mailing address: Institute for Microbial and Biochemical Technology, USDA Forest Products Laboratory, One Gifford Pinchot Dr., Madison, WI 53705. Phone: (608) 231-9528. Fax: (608) 231-9262. E-mail: kehammel{at}wisc.edu

{triangledown} Published ahead of print on 10 October 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, December 2008, p. 7252-7257, Vol. 74, No. 23
0099-2240/08/$08.00+0     doi:10.1128/AEM.01997-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Vanden Wymelenberg, A., Gaskell, J., Mozuch, M., Kersten, P., Sabat, G., Martinez, D., Cullen, D. (2009). Transcriptome and Secretome Analyses of Phanerochaete chrysosporium Reveal Complex Patterns of Gene Expression. Appl. Environ. Microbiol. 75: 4058-4068 [Abstract] [Full Text]  


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