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Applied and Environmental Microbiology, December 2008, p. 7422-7426, Vol. 74, No. 23
0099-2240/08/$08.00+0     doi:10.1128/AEM.01369-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

PBAD-Based Shuttle Vectors for Functional Analysis of Toxic and Highly Regulated Genes in Pseudomonas and Burkholderia spp. and Other Bacteria{triangledown}

Dongru Qiu,1,{ddagger} F. Heath Damron,1,{ddagger} Takehiko Mima,4 Herbert P. Schweizer,4 and Hongwei D. Yu1,2,3*

Departments of Biochemistry and Microbiology,1 Pediatrics, Joan C. Edwards School of Medicine at Marshall University, Huntington, West Virginia 25755-9320,2 Progenesis Technologies, LLC, Bldg. 740, Rm. 4136, Dow Technology Park, 3200 Kanawha Turnpike, South Charleston, West Virginia 25303,3 Department of Microbiology, Immunology and Pathology, Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research, Colorado State University, Fort Collins, Colorado 80523-16904

Received 18 June 2008/ Accepted 6 October 2008

We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the PBAD promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.

* Corresponding author. Mailing address: Robert C. Byrd Biotechnology Science Center, 1 John Marshall Drive, Huntington, WV 25755-9320. Phone: (304) 696-7356. Fax: (304) 696-7207. E-mail: yuh{at}marshall.edu

{triangledown} Published ahead of print on 10 October 2008.

{ddagger} D.Q. and F.H.D. contributed equally to this publication.

Applied and Environmental Microbiology, December 2008, p. 7422-7426, Vol. 74, No. 23
0099-2240/08/$08.00+0     doi:10.1128/AEM.01369-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Damron, F. H., Napper, J., Teter, M. A., Yu, H. D. (2009). Lipotoxin F of Pseudomonas aeruginosa is an AlgU-dependent and alginate-independent outer membrane protein involved in resistance to oxidative stress and adhesion to A549 human lung epithelia. Microbiology 155: 1028-1038 [Abstract] [Full Text]  
  • Damron, F. H., Qiu, D., Yu, H. D. (2009). The Pseudomonas aeruginosa Sensor Kinase KinB Negatively Controls Alginate Production through AlgW-Dependent MucA Proteolysis. J. Bacteriol. 191: 2285-2295 [Abstract] [Full Text]  


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