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Applied and Environmental Microbiology, December 2008, p. 7431-7433, Vol. 74, No. 23
0099-2240/08/$08.00+0     doi:10.1128/AEM.01446-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

The Functional Quality of Soluble Recombinant Polypeptides Produced in Escherichia coli Is Defined by a Wide Conformational Spectrum{triangledown}

Mónica Martínez-Alonso, Nuria González-Montalbán, Elena García-Fruitós, and Antonio Villaverde*

Institute for Biotechnology and Biomedicine, Department of Genetics and Microbiology, Autonomous University of Barcelona, and CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain, Bellaterra, 08193 Barcelona, Spain

Received 27 June 2008/ Accepted 24 September 2008

We have observed that a soluble recombinant green fluorescent protein produced in Escherichia coli occurs in a wide conformational spectrum. This results in differently fluorescent protein fractions in which morphologically diverse soluble aggregates abound. Therefore, the functional quality of soluble versions of aggregation-prone recombinant proteins is defined statistically rather than by the prevalence of a canonical native structure.

* Corresponding author. Mailing address: Institute for Biotechnology and Biomedicine, Autonomous University of Barcelona, Bellaterra, 08193 Barcelona, Spain. Phone: 34 935813086. Fax: 34 935812011. E-mail: avillaverde{at}servet.uab.es

{triangledown} Published ahead of print on 3 October 2008.

Applied and Environmental Microbiology, December 2008, p. 7431-7433, Vol. 74, No. 23
0099-2240/08/$08.00+0     doi:10.1128/AEM.01446-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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