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Applied and Environmental Microbiology, December 2008, p. 7434-7436, Vol. 74, No. 23
0099-2240/08/$08.00+0     doi:10.1128/AEM.01605-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Quantification and Genotyping of Torque Teno Virus at a Wastewater Treatment Plant in Japan{triangledown}

Eiji Haramoto,1* Hiroyuki Katayama,2 and Shinichiro Ohgaki2

Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan,1 Department of Urban Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan2

Received 13 July 2008/ Accepted 24 September 2008

Torque teno virus (TTV) DNA was quantitatively detected in influent and effluent samples collected from a wastewater treatment plant in Japan, with the highest concentration being 4.8 x 104 copies/liter. Genogroup-specific nested PCR demonstrated that TTV of genogroup 3 was the most abundant in wastewater among the five genogroups tested.


* Corresponding author. Mailing address: Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan. Phone: 81-55-220-8725. Fax: 81-55-220-8592. E-mail: eharamoto{at}yamanashi.ac.jp

{triangledown} Published ahead of print on 3 October 2008.


Applied and Environmental Microbiology, December 2008, p. 7434-7436, Vol. 74, No. 23
0099-2240/08/$08.00+0     doi:10.1128/AEM.01605-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.