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Applied and Environmental Microbiology, December 2008, p. 7709-7714, Vol. 74, No. 24
0099-2240/08/$08.00+0     doi:10.1128/AEM.01948-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Transcriptional Analysis of Clostridium beijerinckii NCIMB 8052 and the Hyper-Butanol-Producing Mutant BA101 during the Shift from Acidogenesis to Solventogenesis{triangledown} ,{dagger}

Zhen Shi1* and Hans P. Blaschek1,2

Institute for Genomic Biology,1 Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, Illinois 618012

Received 20 August 2008/ Accepted 2 October 2008

Clostridium beijerinckii is an anaerobic bacterium used for the fermentative production of acetone and butanol. The recent availability of genomic sequence information for C. beijerinckii NCIMB 8052 has allowed for an examination of gene expression during the shift from acidogenesis to solventogenesis over the time course of a batch fermentation using a ca. 500-gene set DNA microarray. The microarray was constructed using a collection of genes which are orthologs of members of gene families previously found to be important to the physiology of C. acetobutylicum ATCC 824. Similar to the onset of solventogenesis in C. acetobutylicum 824, the onset of solventogenesis in C. beijerinckii 8052 was concurrent with the initiation of sporulation. However, forespores and endospores developed more rapidly in C. beijerinckii 8052 than in C. acetobutylicum 824, consistent with the accelerated expression of the sigE- and sigG-regulated genes in C. beijerinckii 8052. The comparison of gene expression patterns and morphological changes in C. beijerinckii 8052 and the hyper-butanol-producing C. beijerinckii strain BA101 indicated that BA101 was less efficient in sporulation and phosphotransferase system-mediated sugar transport than 8052 but that it exhibited elevated expression of several primary metabolic genes and chemotaxis/motility genes.

* Corresponding author. Mailing address: MC-195, 3409 Institute for Genomic Biology, University of Illinois, 1206 W. Gregory Dr., Urbana, IL 61801. Phone: (217) 333-7786. Fax: (217) 333-0508. E-mail: zshi{at}illinois.edu

{triangledown} Published ahead of print on 10 October 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, December 2008, p. 7709-7714, Vol. 74, No. 24
0099-2240/08/$08.00+0     doi:10.1128/AEM.01948-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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