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Applied and Environmental Microbiology, December 2008, p. 7809-7812, Vol. 74, No. 24
0099-2240/08/$08.00+0     doi:10.1128/AEM.00896-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

DNA Microarray Analysis of the Heat Shock Transcriptome of the Obligate Intracytoplasmic Pathogen Rickettsia prowazekii{triangledown} ,{dagger}

Jonathon P. Audia,* Mary C. Patton, and Herbert H. Winkler*

Laboratory of Molecular Biology, Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile, Alabama 36688

Received 20 April 2008/ Accepted 6 October 2008

Here we present the first oligonucleotide DNA microarray analysis of global gene expression changes in the obligate intracytoplasmic pathogen Rickettsia prowazekii using temperature upshift as a model stress condition, and we describe a methodology for isolating highly purified rickettsial RNA. In toto, 23 transcripts were significantly increased by temperature upshift (≥2.0-fold; P < 0.05), and no transcripts demonstrated reproducible decreases. Array results for three heat shock-inducible mRNAs were confirmed using quantitative reverse transcription-PCR.

* Corresponding author. Mailing address: Laboratory of Molecular Biology, Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile, AL 36688. Phone: (251) 460-6929. Fax: (251) 460-7269. E-mail for Jonathon P. Audia: jaudia{at}jaguar1.usouthal.edu. E-mail for Herbert H. Winkler: hwinkler{at}jaguar1.usouthal.edu

{triangledown} Published ahead of print on 24 October 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, December 2008, p. 7809-7812, Vol. 74, No. 24
0099-2240/08/$08.00+0     doi:10.1128/AEM.00896-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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