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Applied and Environmental Microbiology, December 2008, p. 7813-7816, Vol. 74, No. 24
0099-2240/08/$08.00+0     doi:10.1128/AEM.01364-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Use of Flow Cytometry To Monitor Legionella Viability{triangledown}

Séverine Allegra, Françoise Berger, Philippe Berthelot, Florence Grattard, Bruno Pozzetto, and Serge Riffard*

Groupe Immunité des Muqueuses et Agents Pathogènes (GIMAP), EA3064, Faculté de Médecine J. Lisfranc, Université Jean Monnet, Saint-Etienne, France

Received 18 June 2008/ Accepted 7 October 2008

Legionella viability was monitored during heat shock treatment at 70°C by a flow cytometric assay (FCA). After 30 min of treatment, for 6 of the 12 strains tested, the FCA still detected 10 to 25% of cells that were viable but nonculturable (VBNC). These VBNC cells were able to produce ATP and to be resuscitated after culture on amoebae.

* Corresponding author. Mailing address: Département Génie Biologique—Génie de l'Environnement, Institut Universitaire et Technologique (IUT) de Saint-Etienne, Université Jean Monnet, 28 Avenue Léon Jouhaux, 42023 Saint-Etienne cedex 2, France. Phone: 33 4 77 42 14 67. Fax: 33 4 77 42 14 86. E-mail: serge.riffard{at}univ-st-etienne.fr

{triangledown} Published ahead of print on 10 October 2008.

Applied and Environmental Microbiology, December 2008, p. 7813-7816, Vol. 74, No. 24
0099-2240/08/$08.00+0     doi:10.1128/AEM.01364-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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