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Applied and Environmental Microbiology, December 2008, p. 7817-7820, Vol. 74, No. 24
0099-2240/08/$08.00+0     doi:10.1128/AEM.01932-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Enhanced Transformation Efficiency of Recalcitrant Bacillus cereus and Bacillus weihenstephanensis Isolates upon In Vitro Methylation of Plasmid DNA{triangledown}

Masja Nierop Groot,1,3* Frank Nieboer,1,3 and Tjakko Abee2,3

Food Technology Centre, Wageningen University and Research Centre, P.O. Box 17, 6700 AA Wageningen, The Netherlands,1 Laboratory of Food Microbiology, Wageningen University and Research Centre, P.O. Box 8129, 6700 EV Wageningen, The Netherlands,2 TI Food and Nutrition, P.O. Box 557, 6700 AN, Wageningen, The Netherlands3

Received 19 August 2008/ Accepted 17 October 2008

Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested. In vitro methylation of plasmid DNA was used to enhance poor plasmid transfer upon electroporation to recalcitrant strains that carry Sau3AI restriction barriers.

* Corresponding author. Mailing address: Food Technology Centre, Wageningen UR, P.O. Box 17, 6700 AA Wageningen, The Netherlands. Phone: 31-(0)317-480159. Fax: 31-(0)317-483011. E-mail: masja.nieropgroot{at}wur.nl

{triangledown} Published ahead of print on 24 October 2008.

Applied and Environmental Microbiology, December 2008, p. 7817-7820, Vol. 74, No. 24
0099-2240/08/$08.00+0     doi:10.1128/AEM.01932-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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