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Applied and Environmental Microbiology, December 2008, p. 7828-7831, Vol. 74, No. 24
0099-2240/08/$08.00+0     doi:10.1128/AEM.01303-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Cross-Kingdom Amplification Using Bacteria-Specific Primers: Complications for Studies of Coral Microbial Ecology{triangledown}

Julia P. Galkiewicz1 and Christina A. Kellogg2*

University of South Florida, College of Marine Science, 140 7th Ave. S., St. Petersburg, Florida 33701,1 U.S. Geological Survey, 600 4th St. S., St. Petersburg, Florida 337012

Received 9 June 2008/ Accepted 8 October 2008

PCR amplification of pure bacterial DNA is vital to the study of bacterial interactions with corals. Commonly used Bacteria-specific primers 8F and 27F paired with the universal primer 1492R amplify both eukaryotic and prokaryotic rRNA genes. An alternative primer set, 63F/1542R, is suggested to resolve this problem.

* Corresponding author. Mailing address: U.S. Geological Survey, 600 4th St. S., St. Petersburg, FL 33701. Phone: (727) 803-8747. Fax: (727) 803-2031. E-mail: ckellogg{at}usgs.gov

{triangledown} Published ahead of print on 17 October 2008.

Applied and Environmental Microbiology, December 2008, p. 7828-7831, Vol. 74, No. 24
0099-2240/08/$08.00+0     doi:10.1128/AEM.01303-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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