This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oliveira, V. A.
Right arrow Articles by Brandão, R. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oliveira, V. A.
Right arrow Articles by Brandão, R. L.
Agricola
Right arrow Articles by Oliveira, V. A.
Right arrow Articles by Brandão, R. L.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, February 2008, p. 693-701, Vol. 74, No. 3
0099-2240/08/$08.00+0     doi:10.1128/AEM.01729-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Biochemical and Molecular Characterization of Saccharomyces cerevisiae Strains Obtained from Sugar-Cane Juice Fermentations and Their Impact in Cachaça Production{triangledown}

Valdinéia Aparecida Oliveira,1 Maristela Araújo Vicente,1 Luciano Gomes Fietto,1 Ieso de Miranda Castro,1 Maurício Xavier Coutrim,2 Dorit Schüller,3 Henrique Alves,3 Margarida Casal,3 Juliana de Oliveira Santos,4 Leandro Dias Araújo,4 Paulo Henrique Alves da Silva,4 and Rogelio Lopes Brandão1*

Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisas em Ciências Biológicas, Departamento de Farmácia, Escola de Farmácia, Universidade Federal de Ouro Preto,1 Laboratório de Análises Físico-Químicas, Departamento de Química, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, 35.400-000 Ouro Preto, Minas Gerais, Brazil,2 Centro de Biologia, Departamento de Biologia, Universidade do Minho, 4710-057 Braga, Portugal,3 Centro de Ciências Exatas e Tecnológicas, Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, 36.570-000 Viçosa, Minas Gerais, Brazil4

Received 26 July 2007/ Accepted 18 November 2007

Saccharomyces cerevisiae strains from different regions of Minas Gerais, Brazil, were isolated and characterized aiming at the selection of starter yeasts to be used in the production of cachaça, the Brazilian sugar cane spirit. The methodology established took into account the screening for biochemical traits desirable in a yeast cachaça producer, such as no H2S production, high tolerance to ethanol and high temperatures, high fermentative capacity, and the abilities to flocculate and to produce mycocins. Furthermore, the yeasts were exposed to drugs such as 5,5',5"-trifluor-D,L-leucine and cerulenin to isolate those that potentially overproduce higher alcohols and esters. The utilization of a random amplified polymorphic DNA-PCR method with primers based on intron splicing sites flanking regions of the COX1 gene, as well as microsatellite analysis, was not sufficient to achieve good differentiation among selected strains. In contrast, karyotype analysis allowed a clear distinction among all strains. Two selected strains were experimentally evaluated as cachaça producers. The results suggest that the selection of strains as fermentation starters requires the combined use of biochemical and molecular criteria to ensure the isolation and identification of strains with potential characteristics to produce cachaça with a higher quality standard.

* Corresponding author. Mailing address: Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisas em Ciências Biológicas, ICEB II, Universidade Federal de Ouro Preto, Campus do Morro do Cruzeiro, 35.400-000 Ouro Preto, Minas Gerais, Brazil. Phone: 55 31 3559 1723. Fax: 55 31 3559 1680. E-mail: rlbrand{at}nupeb.ufop.br

{triangledown} Published ahead of print on 7 December 2007.

Applied and Environmental Microbiology, February 2008, p. 693-701, Vol. 74, No. 3
0099-2240/08/$08.00+0     doi:10.1128/AEM.01729-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»