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Applied and Environmental Microbiology, February 2008, p. 702-707, Vol. 74, No. 3
0099-2240/08/$08.00+0     doi:10.1128/AEM.01805-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Effects of ADH2 Overexpression in Saccharomyces bayanus during Alcoholic Fermentation{triangledown}

Oscar Maestre,1 Teresa García-Martínez,1 Rafael A. Peinado,2 and Juan C. Mauricio1*

Department of Microbiology, University of Córdoba, Edificio Severo Ochoa, Campus Universitario de Rabanales, 14014 Cordóba, Spain,1 Department of Agricultural Chemistry, University of Córdoba, Edificio Marie Curie, Campus Universitario de Rabanales, 14014 Córdoba, Spain2

Received 3 August 2007/ Accepted 18 November 2007

The effect of overexpression of the gene ADH2 on metabolic and biological activity in Saccharomyces bayanus V5 during alcoholic fermentation has been evaluated. This gene is known to encode alcohol dehydrogenase II (ADH II). During the biological aging of sherry wines, where yeasts have to grow on ethanol owing to the absence of glucose, this isoenzyme plays a prominent role by converting the ethanol into acetaldehyde and producing NADH in the process. Overexpression of the gene ADH2 during alcoholic fermentation has no effect on the proteomic profile or the net production of some metabolites associated with glycolysis and alcoholic fermentation such as ethanol, acetaldehyde, and glycerol. However, it affects indirectly glucose and ammonium uptakes, cell growth, and intracellular redox potential, which lead to an altered metabolome. The increased contents in acetoin, acetic acid, and L-proline present in the fermentation medium under these conditions can be ascribed to detoxification by removal of excess acetaldehyde and the need to restore and maintain the intracellular redox potential balance.

* Corresponding author. Mailing address: Departamento de Microbiología, Universidad de Córdoba, Edificio Severo Ochoa, Campus Universitario de Rabanales, 14014 Córdoba, Spain. Phone: 34 957218640. Fax: 34 957218650. E-mail: mi1gamaj{at}uco.es

{triangledown} Published ahead of print on 7 December 2007.

Applied and Environmental Microbiology, February 2008, p. 702-707, Vol. 74, No. 3
0099-2240/08/$08.00+0     doi:10.1128/AEM.01805-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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