This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cutter, M. R.
Right arrow Articles by Stroot, P. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cutter, M. R.
Right arrow Articles by Stroot, P. G.
Agricola
Right arrow Articles by Cutter, M. R.
Right arrow Articles by Stroot, P. G.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, February 2008, p. 901-903, Vol. 74, No. 3
0099-2240/08/$08.00+0     doi:10.1128/AEM.01899-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

REPORT

Determination of Specific Growth Rate by Measurement of Specific Rate of Ribosome Synthesis in Growing and Nongrowing Cultures of Acinetobacter calcoaceticus{triangledown} ,{dagger}

Matthew R. Cutter and Peter G. Stroot*

Department of Civil and Environmental Engineering, University of South Florida, 4202 East Fowler Ave, ENB118, Tampa, Florida 33620-5350

Received 17 August 2007/ Accepted 29 November 2007

ABSTRACT

RT-RiboSyn measures the specific rate of ribosome synthesis in distinct microbial populations by measuring the generation rate of precursor 16S rRNA relative to that of mature 16S rRNA when precursor 16S rRNA processing is inhibited. Good agreement was demonstrated between specific rate of ribosome synthesis and specific growth rate of Acinetobacter calcoaceticus.


* Corresponding author. Mailing address: 4202 East Fowler Ave., ENB118, Tampa, FL 33620-5350. Phone: (813) 396-9323. Fax: (813) 974-9341. E-mail: pstroot{at}eng.usf.edu

FOOTNOTES

{triangledown} Published ahead of print on 14 December 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.


Applied and Environmental Microbiology, February 2008, p. 901-903, Vol. 74, No. 3
0099-2240/08/$08.00+0     doi:10.1128/AEM.01899-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:

  • Lu, T., Stroot, P. G., Oerther, D. B. (2009). Reverse Transcription of 16S rRNA To Monitor Ribosome-Synthesizing Bacterial Populations in the Environment. Appl. Environ. Microbiol. 75: 4589-4598 [Abstract] [Full Text]