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Applied and Environmental Microbiology, February 2008, p. 1058-1063, Vol. 74, No. 4
0099-2240/08/$08.00+0     doi:10.1128/AEM.02342-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Poly(3-Hydroxybutyrate) (PHB) Depolymerase PhaZa1 Is Involved in Mobilization of Accumulated PHB in Ralstonia eutropha H16{triangledown}

Keiichi Uchino,1,2 Terumi Saito,2 and Dieter Jendrossek1*

Institut für Mikrobiologie, Universität Stuttgart, 70550 Stuttgart, Germany,1 Laboratory of Molecular Microbiology, Kanagawa University, Kanagawa 259-1293, Japan2

Received 17 October 2007/ Accepted 10 December 2007

The recently finished genome sequence of Ralstonia eutropha H16 harbors nine genes that are thought to encode functions for intracellular depolymerization (mobilization) of storage poly(3-hydroxybutyrate) (PHB). Based on amino acid similarities, the gene products belong to four classes (PhaZa1 to PhaZa5, PhaZb, PhaZc, and PhaZd1/PhaZd2). However, convincing direct evidence for the in vivo roles of the gene products is poor. In this study, we selected four candidate genes (phaZa1, phaZb, phaZc, and phaZd1) representing the four classes and investigated the physiological function of the gene products (i) with recombinant Escherichia coli strains and (ii) with R. eutropha null mutants. Evidence for weak but significant PHB depolymerase activity was obtained only for PhaZa1. The physiological roles of the other potential PHB depolymerases remain uncertain.

* Corresponding author. Mailing address: Institut für Mikrobiologie, Universität Stuttgart, Allmandring 31, 70550 Stuttgart, Germany. Phone: 49-711-685-65483. Fax: 49-711-685-65725. E-mail: dieter.jendrossek{at}imb.uni-stuttgart.de

{triangledown} Published ahead of print on 21 December 2007.

Applied and Environmental Microbiology, February 2008, p. 1058-1063, Vol. 74, No. 4
0099-2240/08/$08.00+0     doi:10.1128/AEM.02342-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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