AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Other Versions of this Article:
AEM.02185-07v1
74/4/1250    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rizzi, A.
Right arrow Articles by Daffonchio, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rizzi, A.
Right arrow Articles by Daffonchio, D.
Agricola
Right arrow Articles by Rizzi, A.
Right arrow Articles by Daffonchio, D.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, February 2008, p. 1250-1254, Vol. 74, No. 4
0099-2240/08/$08.00+0     doi:10.1128/AEM.02185-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Strategy for In Situ Detection of Natural Transformation-Based Horizontal Gene Transfer Events{triangledown} ,{dagger}

Aurora Rizzi,1 Alessandra Pontiroli,1,2 Lorenzo Brusetti,1 Sara Borin,1 Claudia Sorlini,1 Alessandro Abruzzese,3 Gian Attilio Sacchi,3 Timothy M. Vogel,2 Pascal Simonet,2 Marco Bazzicalupo,4 Kaare Magne Nielsen,5,6 Jean-Michel Monier,2 and Daniele Daffonchio1*

Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche (DISTAM),1 Dipartimento di Produzione Vegetale (DIPROVE), Università degli Studi di Milano, 20133 Milan, Italy,3 Environmental Microbial Genomics Group, Laboratoire Ampère, UMR CNRS 5005, Ecole Centrale de Lyon, Université de Lyon, 69134 Ecully Cedex, France,2 Dipartimento di Biologia Animale e Genetica (DBAG), Università degli Studi di Firenze, 50125 Florence, Italy,4 Department of Pharmacy, University of Tromso,5 Norwegian Institute of Gene Ecology, Research Park, 9294 Tromso, Norway6

Received 25 September 2007/ Accepted 18 December 2007

A strategy is described that enables the in situ detection of natural transformation in Acinetobacter baylyi BD413 by the expression of a green fluorescent protein. Microscale detection of bacterial transformants growing on plant tissues was shown by fluorescence microscopy and indicated that cultivation-based selection of transformants on antibiotic-containing agar plates underestimates transformation frequencies.

* Corresponding author. Mailing address: Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche (DISTAM), Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy. Phone: 39-0250319117. Fax: 39-0250319238. E-mail: daniele.daffonchio{at}unimi.it

{triangledown} Published ahead of print on 28 December 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, February 2008, p. 1250-1254, Vol. 74, No. 4
0099-2240/08/$08.00+0     doi:10.1128/AEM.02185-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2008 by the American Society for Microbiology. All rights reserved.