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Applied and Environmental Microbiology, February 2008, p. 1264-1267, Vol. 74, No. 4
0099-2240/08/$08.00+0     doi:10.1128/AEM.01585-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Role of galK and galM in Galactose Metabolism by Streptococcus thermophilus{triangledown}

Katy Vaillancourt,1 Nathalie Bédard,1 Christian Bart,1 Mélanie Tessier,1 Gilles Robitaille,2 Nathalie Turgeon,1 Michel Frenette,1 Sylvain Moineau,1 and Christian Vadeboncoeur1*

Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, and Département de Biochimie et de Microbiologie, Faculté des Sciences et de Génie, Université Laval, Quebec City, Quebec, Canada G1K 7P4,1 Food Research and Development Centre, Agriculture and Agri-Food Canada, St. Hyacinthe, Quebec, Canada J2S 8E32

Received 12 July 2007/ Accepted 18 November 2007

Streptococcus thermophilus is unable to metabolize the galactose moiety of lactose. In this paper, we show that a transformant of S. thermophilus SMQ-301 expressing Streptococcus salivarius galK and galM was able to grow on galactose and expelled at least twofold less galactose into the medium during growth on lactose.

* Corresponding author. Mailing address: Département de Biochimie et de Microbiologie, Faculté des Sciences et de Génie, Université Laval, Quebec City, Quebec, Canada G1K 7P4. Phone: (418) 656-2319. Fax: (418) 656-2861. E-mail: Christian.Vadeboncoeur{at}bcm.ulaval.ca

{triangledown} Published ahead of print on 7 December 2007.

Applied and Environmental Microbiology, February 2008, p. 1264-1267, Vol. 74, No. 4
0099-2240/08/$08.00+0     doi:10.1128/AEM.01585-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Robitaille, G., Tremblay, A., Moineau, S., St-Gelais, D., Vadeboncoeur, C., Britten, M. (2009). Fat-free yogurt made using a galactose-positive exopolysaccharide-producing recombinant strain of Streptococcus thermophilus. J DAIRY SCI 92: 477-482 [Abstract] [Full Text]  


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