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Applied and Environmental Microbiology, February 2008, p. 1273-1275, Vol. 74, No. 4
0099-2240/08/$08.00+0     doi:10.1128/AEM.02347-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Sensitivity of an Acinetobacter baylyi mpl Mutant to DNA Damage

Adityarup Chakravorty, Martha Klovstad, Greg Peterson, Robin E. Lindeman,^¶ and Leslie A. Gregg-Jolly^*

Department of Biology, Grinnell College, Grinnell, Iowa 50112

Received 17 October 2007/ Accepted 7 December 2007

A mutant derived from Acinetobacter baylyi ADP1 was isolated from a screen for genes involved in the response to DNA damage. This derivative has an insertion in the mpl gene which encodes a peptidoglycan-recycling protein. We demonstrate that the insertion renders cells sensitive to mitomycin C and to UV.

Published ahead of print on 14 December 2007.

Present address: Department of Biology, University of Rochester. Rochester, NY.

Present address: Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ.

Present address: University of Minnesota Medical School, Minneapolis, MN.

^¶ Present address: Laboratory of Genetics, University of Wisconsin-Madison, Madison, WI.