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Functional-Genomics-Based Identification and Characterization of Open Reading Frames Encoding -Glucoside-Processing Enzymes in the Hyperthermophilic Archaeon Pyrococcus furiosus ^,

Donald A. Comfort, Chung-Jung Chou, Shannon B. Conners, Amy L. VanFossen, and Robert M. Kelly^*

Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27695-7905

Received 21 August 2007/ Accepted 8 December 2007

Bioinformatics analysis and transcriptional response information for Pyrococcus furiosus grown on -glucans led to the identification of a novel isomaltase (PF0132) representing a new glycoside hydrolase (GH) family, a novel GH57 β-amylase (PF0870), and an extracellular starch-binding protein (1,141 amino acids; PF1109-PF1110), in addition to several other putative -glucan-processing enzymes.

Published ahead of print on 21 December 2007.

Supplemental material for this article may be found at http://aem.asm.org/.

Present address: Wyeth Pharmaceuticals, Sanford, NC.

Present address: SAS Corporation, Cary, NC.