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Applied and Environmental Microbiology, February 2008, p. 1294-1298, Vol. 74, No. 4
0099-2240/08/$08.00+0     doi:10.1128/AEM.02660-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Determination of Glycosyltransferase Specificities for the Escherichia coli O111 O Antigen by a Generic Approach{triangledown} ,{dagger}

Gordon Stevenson, Manuela Dieckelmann, and Peter R. Reeves*

School of Molecular and Microbial Biosciences, University of Sydney, Sydney, New South Wales 2006, Australia

Received 23 November 2007/ Accepted 27 November 2007

We describe a bacterial strain developed to facilitate the determination of glycosyltransferase (GT) specificities for O antigens of known structure and gene cluster sequence. For proof of principle for the approach, the strain was used to determine the specificity of the Escherichia coli O111 O-antigen GT genes.

* Corresponding author. Mailing address: School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW 2006, Australia. Phone: 612-93512536. Fax: 612-93514571. E-mail: reeves{at}angis.usyd.edu.au

{triangledown} Published ahead of print on 21 December 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, February 2008, p. 1294-1298, Vol. 74, No. 4
0099-2240/08/$08.00+0     doi:10.1128/AEM.02660-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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