This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Uroz, S.
Right arrow Articles by Dessaux, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Uroz, S.
Right arrow Articles by Dessaux, Y.
Agricola
Right arrow Articles by Uroz, S.
Right arrow Articles by Dessaux, Y.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2008, p. 1357-1366, Vol. 74, No. 5
0099-2240/08/$08.00+0     doi:10.1128/AEM.02014-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

A Rhodococcus qsdA-Encoded Enzyme Defines a Novel Class of Large-Spectrum Quorum-Quenching Lactonases{triangledown} ,{dagger}

Stéphane Uroz,1,2,{ddagger} Phil M. Oger,2* Emilie Chapelle,1 Marie-Thérèse Adeline,3 Denis Faure,1 and Yves Dessaux1

Interactions Plantes et Microorganismes de la Rhizosphère, Institut des Sciences du Végétal, CNRS, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex,1 Laboratoire de Sciences de la Terre, UMR CNRS 5570, Ecole Normale Supérieure, 46, Allée d'Italie, 69364 Lyon Cedex,2 Institut de Chimie des Substances Naturelles, CNRS, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France3

Received 3 September 2007/ Accepted 22 December 2007

A gene involved in N-acyl homoserine lactone (N-AHSL) degradation was identified by screening a genomic library of Rhodococcus erythropolis strain W2. This gene, named qsdA (for quorum-sensing signal degradation), encodes an N-AHSL lactonase unrelated to the two previously characterized N-AHSL-degrading enzymes, i.e., the lactonase AiiA and the amidohydrolase AiiD. QsdA is related to phosphotriesterases and constitutes the reference of a novel class of N-AHSL degradation enzymes. It confers the ability to inactivate N-AHSLs with an acyl chain ranging from C6 to C14, with or without substitution at carbon 3. Screening of a collection of 15 Rhodococcus strains and strains closely related to this genus clearly highlighted the relationship between the ability to degrade N-AHSLs and the presence of the qsdA gene in Rhodococcus. Bacteria harboring the qsdA gene interfere very efficiently with quorum-sensing-regulated functions, demonstrating that qsdA is a valuable tool for developing quorum-quenching procedures.

* Corresponding author. Mailing address: Laboratoire de Sciences de la Terre, UMR CNRS 5570, Ecole Normale Supérieure, 46, Allée d'Italie, 69364 Lyon Cedex, France. Phone: (33) 4 72 72 87 92. Fax: (33) 4 72 72 86 77. E-mail: poger{at}ens-lyon.fr

{triangledown} Published ahead of print on 11 January 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} Present address: Interactions Arbres Microorganismes, INRA, 54280 Champenoux, France.

Applied and Environmental Microbiology, March 2008, p. 1357-1366, Vol. 74, No. 5
0099-2240/08/$08.00+0     doi:10.1128/AEM.02014-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schipper, C., Hornung, C., Bijtenhoorn, P., Quitschau, M., Grond, S., Streit, W. R. (2009). Metagenome-Derived Clones Encoding Two Novel Lactonase Family Proteins Involved in Biofilm Inhibition in Pseudomonas aeruginosa. Appl. Environ. Microbiol. 75: 224-233 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»