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Applied and Environmental Microbiology, March 2008, p. 1367-1375, Vol. 74, No. 5
0099-2240/08/$08.00+0     doi:10.1128/AEM.02261-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Role of Campylobacter jejuni Respiratory Oxidases and Reductases in Host Colonization{triangledown}

Rebecca A. Weingarten,1 Jesse L. Grimes,2 and Jonathan W. Olson1*

Department of Microbiology, North Carolina State University, Raleigh, North Carolina,1 Department of Poultry Science, North Carolina State University, Raleigh, North Carolina2

Received 4 October 2007/ Accepted 24 December 2007

Campylobacter jejuni is the leading cause of human food-borne bacterial gastroenteritis. The C. jejuni genome sequence predicts a branched electron transport chain capable of utilizing multiple electron acceptors. Mutants were constructed by disrupting the coding regions of the respiratory enzymes nitrate reductase (napA::Cm), nitrite reductase (nrfA::Cm), dimethyl sulfoxide, and trimethylamine N-oxide reductase (termed Cj0264::Cm) and the two terminal oxidases, a cyanide-insensitive oxidase (cydA::Cm) and cbb3-type oxidase (ccoN::Cm). Each strain was characterized for the loss of the associated enzymatic function in vitro. The strains were then inoculated into 1-week-old chicks, and the cecal contents were assayed for the presence of C. jejuni 2 weeks postinoculation. cydA::Cm and Cj0264c::Cm strains colonized as well as the wild type; napA::Cm and nrfA::Cm strains colonized at levels significantly lower than the wild type. The ccoN::Cm strain was unable to colonize the chicken; no colonies were recovered at the end of the experiment. While there appears to be a role for anaerobic respiration in host colonization, oxygen is the most important respiratory acceptor for C. jejuni in the chicken cecum.

* Corresponding author. Mailing address: Department of Microbiology, North Carolina State University, Campus Box 7615, Raleigh, NC 27695. Phone: (919) 515-7860. Fax: (919) 515-7867. E-mail: jwolson{at}ncsu.edu

{triangledown} Published ahead of print on 11 January 2008.

Applied and Environmental Microbiology, March 2008, p. 1367-1375, Vol. 74, No. 5
0099-2240/08/$08.00+0     doi:10.1128/AEM.02261-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gonzalez, M., Hakkinen, M., Rautelin, H., Hanninen, M.-L. (2009). Bovine Campylobacter jejuni Strains Differ from Human and Chicken Strains in an Analysis of Certain Molecular Genetic Markers. Appl. Environ. Microbiol. 75: 1208-1210 [Abstract] [Full Text]  


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