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Applied and Environmental Microbiology, March 2008, p. 1428-1435, Vol. 74, No. 5
0099-2240/08/$08.00+0 doi:10.1128/AEM.02039-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Surface Response of Fluorine Polymer-Incorporated Resin Composites to Cariogenic Biofilm Adherence
,
Mariko Gyo,1*
Toru Nikaido,1
Koichi Okada,3
Junichi Yamauchi,3
Junji Tagami,1,2 and
Khairul Matin1,2
Cariology and Operative Dentistry, Department of Restorative Sciences, Graduate School, Tokyo Medical and Dental University, 5-45 Yushima 1-chome, Bunkyo-ku, Tokyo 113-8549, Japan,1 Center of Excellence Program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and Bone, Tokyo Medical and Dental University, 5-45 Yushima 1-chome, Bunkyo-ku, Tokyo 113-8549, Japan,2 Kuraray Medical, Ote Center Building, 1-1-3 Otemachi, Chiyoda-ku, Tokyo 110-0004, Japan3
Received 6 September 2007/ Accepted 30 December 2007
Experimental resin composites with incorporated polytetrafluoroethylene (PTFE) particles were developed, which theoretically could improve the surface properties of the materials, including the inhibition of bacterial adherence. To assess the surface properties in relation to biofilm formation and detachment, 23.1% (wt/wt) linear PTFE particles (FL-30) and cross-linked PTFE particles (FC-30) were incorporated into pure resin composites. Pure PTFE plates and pure resin composites without PTFE (F-0) were used as control specimens. Sucrose-dependent Streptococcus mutans biofilms were formed on the specimen blocks inside an oral biofilm reactor for various time periods and analyzed with or without application of driving forces. In addition, water contact angles and surface roughness were measured. The water contact angles of FL-30 (61.2°) and FC-30 (65.8°) were larger than that of F-0 (48.5°). The largest contact angle (107°) was detected on pure PTFE plates. However, the surfaces of FL-30, FC-30, and pure PTFE plates were rougher than that of F-0. Although the surface properties of the materials differed in terms of contact angles and roughness, these factors seemed not to affect biofilm formation on the surfaces within 5 h. Pure PTFE plates harbored almost the same amounts of biofilm as F-0. However, when a very strong driving force was applied, it was clear that there were significantly smaller amounts of biofilms retained on pure PTFE plates, which showed contact angles much higher than those of the other materials. Hydrophobicity of the resin composite was improved by incorporation of PTFE fillers. However, surface resistance against biofilm formation was not improved.
* Corresponding author. Mailing address: Cariology and Operative Dentistry, Department of Restorative Sciences, Graduate School, Tokyo Medical and Dental University, 5-45 Yushima 1-chome, Bunkyo-ku, Tokyo 113-8549, Japan. Phone: 81-3-5803-5483. Fax: 81-3-5803-0195. E-mail: gyo2167{at}yahoo.co.jp
Published ahead of print on 11 January 2008.
Supplemental material for this article may be found at http://aem.asm.org/.
Applied and Environmental Microbiology, March 2008, p. 1428-1435, Vol. 74, No. 5
0099-2240/08/$08.00+0 doi:10.1128/AEM.02039-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.
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