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Applied and Environmental Microbiology, March 2008, p. 1656-1659, Vol. 74, No. 5
0099-2240/08/$08.00+0     doi:10.1128/AEM.02127-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Prevalence and Abundance of Uncultivated Megasphaera-Like Bacteria in the Human Vaginal Environment{triangledown}

Marcela Zozaya-Hinchliffe,1 David H. Martin,3 and Michael J. Ferris1,2*

Department of Pediatrics,2 Department of Internal Medicine, Louisiana State University Health Sciences Center,3 The Research Institute for Children, New Orleans, Louisiana1

Received 17 September 2007/ Accepted 7 January 2008

Cultivation-independent analysis of 16S rRNA gene sequences in vaginal samples revealed two previously unrecognized, uncultivated Megasphaera-like phylotypes. Phylogenetic analysis and environmental distribution suggest that these Megasphaera types may be unique to the vaginal environment. Quantitative PCR suggests that both phylotypes are present in higher concentrations in women with bacterial vaginosis.

* Corresponding author. Mailing address: Research and Education Bldg., Children's Hospital, 200 Henry Clay Ave., New Orleans, LA 70118. Phone: (504) 896-5379. Fax: (504) 896-9413. E-mail: mferris{at}chnola-research.org

{triangledown} Published ahead of print on 18 January 2008.

Applied and Environmental Microbiology, March 2008, p. 1656-1659, Vol. 74, No. 5
0099-2240/08/$08.00+0     doi:10.1128/AEM.02127-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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