AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
AEM.02403-07v1
74/5/1660    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bru, D.
Right arrow Articles by Philippot, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bru, D.
Right arrow Articles by Philippot, L.
Agricola
Right arrow Articles by Bru, D.
Right arrow Articles by Philippot, L.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2008, p. 1660-1663, Vol. 74, No. 5
0099-2240/08/$08.00+0     doi:10.1128/AEM.02403-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Quantification of the Detrimental Effect of a Single Primer-Template Mismatch by Real-Time PCR Using the 16S rRNA Gene as an Example{triangledown}

D. Bru, F. Martin-Laurent, and L. Philippot*

INRA, University of Burgundy, Soil and Environmental Microbiology, CMSE, 17 Rue Sully, B.P. 86510, 21065 Dijon Cedex, France

Received 25 October 2007/ Accepted 3 January 2008

We investigated the effects of internal primer-template mismatches on the efficiency of PCR amplification using the 16S rRNA gene as the model template DNA. We observed that the presence of a single mismatch in the second half of the primer extension sequence can result in an underestimation of up to 1,000-fold of the gene copy number, depending on the primer and position of the mismatch.

* Corresponding author. Mailing address: INRA, University of Burgundy, Soil and Environmental Microbiology, CMSE, 17 Rue Sully, B.P. 86510, 21065 Dijon Cedex, France. Phone: 33 3 80 69 33 46. Fax: 33 3 80 69 32 24. E-mail: philippo{at}dijon.inra.fr

{triangledown} Published ahead of print on 11 January 2008.

Applied and Environmental Microbiology, March 2008, p. 1660-1663, Vol. 74, No. 5
0099-2240/08/$08.00+0     doi:10.1128/AEM.02403-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2008 by the American Society for Microbiology. All rights reserved.