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 Previous Article

Applied and Environmental Microbiology, March 2008, p. 1671-1675, Vol. 74, No. 5
0099-2240/08/$08.00+0     doi:10.1128/AEM.01619-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Applicability of Phylogenetic Methods for Characterizing the Public Health Significance of Verocytotoxin-Producing Escherichia coli Strains{triangledown}

Kim Ziebell,1 Paulina Konczy,1 Irene Yong,1 Shelley Frost,1 Mariola Mascarenhas,1 Andrew M. Kropinski,1 Thomas S. Whittam,2 Susan C. Read,1 and Mohamed A. Karmali1*

Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario N1G 3W4, Canada,1 Microbial Evolution Laboratory, National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 488242

Received 16 July 2007/ Accepted 20 December 2007

Two phylogenetic methods (multilocus sequence typing [MLST] and a multiplex PCR) were investigated to determine whether phylogenetic classification of verocytotoxin-producing Escherichia coli serotypes correlates with their classification into groups (seropathotypes A to E) based on their relative incidence in human disease and on their association with outbreaks and serious complications. MLST was able to separate 96% of seropathotype D and E serotypes from those that cause serious disease (seropathotypes A to C), whereas the multiplex PCR lacked this level of seropathotype discrimination.

* Corresponding author. Mailing address: Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, 110 Stone Rd. W, Guelph, Ontario N1G 3W4, Canada. Phone: (519) 826-2606. Fax: (519) 822-2280. E-mail: Mohamed_Karmali{at}phac-aspc.gc.ca

{triangledown} Published ahead of print on 28 December 2007.

Applied and Environmental Microbiology, March 2008, p. 1671-1675, Vol. 74, No. 5
0099-2240/08/$08.00+0     doi:10.1128/AEM.01619-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Konczy, P., Ziebell, K., Mascarenhas, M., Choi, A., Michaud, C., Kropinski, A. M., Whittam, T. S., Wickham, M., Finlay, B., Karmali, M. A. (2008). Genomic O Island 122, Locus for Enterocyte Effacement, and the Evolution of Virulent Verocytotoxin-Producing Escherichia coli. J. Bacteriol. 190: 5832-5840 [Abstract] [Full Text]  


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