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Applied and Environmental Microbiology, March 2008, p. 1941-1944, Vol. 74, No. 6
0099-2240/08/$08.00+0     doi:10.1128/AEM.01658-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Quantitative Detection of Perchlorate-Reducing Bacteria by Real-Time PCR Targeting the Perchlorate Reductase Gene{triangledown}

Mamie Nozawa-Inoue,1* Mercy Jien,1 Nicholas S. Hamilton,1 Valley Stewart,2 Kate M. Scow,1 and Krassimira R. Hristova1

Department of Land, Air, and Water Resources, University of California, Davis,1 Section of Microbiology, University of California, Davis, Davis, California2

Received 19 July 2007/ Accepted 19 January 2008

A quantitative real-time PCR assay targeting the pcrA gene, encoding the catalytic subunit of perchlorate reductase, detected pcrA genes from perchlorate-reducing bacteria in three different genera and from soil microbial communities. Partial pcrA sequences indicated differences in the composition of perchlorate-reducing bacterial communities following exposure to different electron donors.

* Corresponding author. Mailing address: Department of Land, Air, and Water Resources, University of California, Davis, One Shields Avenue, Davis, CA 95616. Phone: (530) 752-0146. Fax: (530) 752-1552. E-mail: minoue{at}ucdavis.edu

{triangledown} Published ahead of print on 1 February 2008.

Applied and Environmental Microbiology, March 2008, p. 1941-1944, Vol. 74, No. 6
0099-2240/08/$08.00+0     doi:10.1128/AEM.01658-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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