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Applied and Environmental Microbiology, March 2008, p. 1945-1949, Vol. 74, No. 6
0099-2240/08/$08.00+0     doi:10.1128/AEM.01044-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Protozoan Migration in Bent Microfluidic Channels{triangledown}

Wei Wang,1 Leslie M. Shor,1,2 Eugene J. LeBoeuf,1,2 John P. Wikswo,2,3 Gary L. Taghon,4 and David S. Kosson1,2*

Department of Civil and Environmental Engineering, Vanderbilt University,1 Vanderbilt Institute for Integrative Biosystems Research and Education,2 Departments of Biomedical Engineering, Molecular Physiology & Biophysics, and Physics & Astronomy, Vanderbilt University, Nashville, Tennessee,3 Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey4

Received 10 May 2007/ Accepted 20 December 2007

Microfluidic devices permit direct observation of microbial behavior in defined microstructured settings. Here, the swimming speed and dispersal of individual marine ciliates in straight and bent microfluidic channels were quantified. The dispersal rate and swimming speed increased with channel width, decreased with protozoan size, and was significantly impacted by the channel turning angle.

* Corresponding author. Mailing address: Department of Civil and Environmental Engineering, Vanderbilt University, Box 1831 Station B, Nashville, TN 37235. Phone: (615) 322-1064. Fax: (615) 322-3365. E-mail: david.kosson{at}vanderbilt.edu

{triangledown} Published ahead of print on 28 December 2007.

Applied and Environmental Microbiology, March 2008, p. 1945-1949, Vol. 74, No. 6
0099-2240/08/$08.00+0     doi:10.1128/AEM.01044-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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