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Traceability of Asian Matsutake, Specialty Mushrooms Produced by the Ectomycorrhizal Basidiomycete Tricholoma matsutake, on the Basis of Retroelement-Based DNA Markers

Hitoshi Murata,^1* Katsuhiko Babasaki,¹ Tomoki Saegusa,² Kenji Takemoto,^2, Akiyoshi Yamada,³ and Akira Ohta⁴

Department of Applied Microbiology and Mushroom Sciences, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan,¹ Central Customs Laboratory, Ministry of Finance, Kashiwa-shi, Chiba 277-0882, Japan,² Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, Minami-minowa, Nagano 399-4598, Japan,³ Shiga Forest Research Center, Yasu, Shiga 520-2321, Japan⁴

Received 26 October 2007/ Accepted 29 January 2008

The ectomycorrhizal basidiomycete Tricholoma matsutake produces commercially valuable fruit bodies, matsutake, in forests. Here we report a PCR system targeting retroelement integration sites to differentiate among individual Asian isolates of T. matsutake based on their geographical origins, such as Japan, the area of South Korea through North Korea, the northeastern provinces of China, and the area of the southwestern provinces of China through Bhutan. The overall misjudgment rate of the analytical system was approximately 5% based on 95 samples of T. matsutake examined including those from cultures and from commodities. We also provide evidence that T. matsutake isolates grown throughout the Far East, including the northeastern provinces of China, are closely related to each other while distinct from those in the area of the southwestern provinces of China through Bhutan. The method allows us to trace back geographical origins of Asian matsutake, thus contributing to food safety, appropriate tariffs, and proper price setting.

Published ahead of print on 15 February 2008.

Present address: Customs Laboratory Clearance Division of Tokyo Customs, Koto-ku Aomi 2-56, Tokyo 135-8615, Japan.