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Applied and Environmental Microbiology, April 2008, p. 2032-2036, Vol. 74, No. 7
0099-2240/08/$08.00+0     doi:10.1128/AEM.02337-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Relationship between Solute and Matric Potential Stress, Temperature, Growth, and FUM1 Gene Expression in Two Fusarium verticillioides Strains from Spain{triangledown}

Miguel Jurado,1 Patricia Marín,1 Naresh Magan,2 and Maria Teresa González-Jaén1*

Department of Genetics, University Complutense Madrid, Jose Antonio Novais 2, 28040 Madrid, Spain,1 Applied Mycology Group, Cranfield Health, Cranfield University, Silsoe, Bedford MK45 4DT, United Kingdom2

Received 16 October 2007/ Accepted 1 February 2008

The objective of this work was to study the effect of ecophysiological factors on fumonisin gene expression and growth in Fusarium verticillioides. The effects of ionic and nonionic solute water potentials, matric potential, and temperature on in vitro mycelial growth rates and on expression of the FUM1 gene, involved in fumonisin biosynthesis, were examined. FUM1 transcript levels were quantified using a specific real-time reverse transcription-PCR (RT-PCR) protocol. Low temperature and water stress reduced fungal growth. Water stress increased FUM1 transcript levels, especially in the case of stress caused by nonionic solute. The temporal kinetic assays showed that water stress had opposite effects on fungal growth versus FUM1 expression. These results indicate that water stress may be an important factor for fumonisin accumulation, particularly in the later phases of maize colonization when water availability decreases. The quantitative RT-PCR methods described here provide a valuable tool for investigating the ecophysiological basis for fumonisin gene expression and ultimately may lead to more effective control strategies for this important mycotoxigenic pathogen.

* Corresponding author. Mailing address: Department of Genetics, University Complutense Madrid, Jose Antonio Novais 2, 28040 Madrid, Spain. Phone: 34 913 944 830. Fax: 34 913 944 844. E-mail: tegonja{at}bio.ucm.es

{triangledown} Published ahead of print on 8 February 2008.

Applied and Environmental Microbiology, April 2008, p. 2032-2036, Vol. 74, No. 7
0099-2240/08/$08.00+0     doi:10.1128/AEM.02337-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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