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Applied and Environmental Microbiology, April 2008, p. 2244-2247, Vol. 74, No. 7
0099-2240/08/$08.00+0     doi:10.1128/AEM.01144-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Enzymatic Permeabilization of the Thecate Dinoflagellate Alexandrium minutum (Dinophyceae) Yields Detection of Intracellularly Associated Bacteria via Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization{triangledown}

Lucía Palacios and Irma Marín*

Departamento de Biología Molecular, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Cantoblanco, Madrid, Spain

Received 22 May 2007/ Accepted 30 January 2008

The enzymatic permeabilization procedure described here allows the detection of intracellular bacteria in the thecate dinoflagellate Alexandrium minutum by using catalyzed reporter deposition-fluorescence in situ hybridization. The combined use of propidium iodide and calcofluor for confocal laser scanning microscopy, together with general and specific fluorescent bacterial probes, demonstrated the intracellular presence of bacteria, including members of the phylum Bacteroidetes.

* Corresponding author. Mailing address: Departamento de Biología Molecular, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Cantoblanco, Madrid, Spain. Phone: 0034914978077. Fax: 0034914978300. E-mail: imarin{at}cbm.uam.es

{triangledown} Published ahead of print on 8 February 2008.

Applied and Environmental Microbiology, April 2008, p. 2244-2247, Vol. 74, No. 7
0099-2240/08/$08.00+0     doi:10.1128/AEM.01144-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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