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 Previous Article

Applied and Environmental Microbiology, April 2008, p. 2254-2258, Vol. 74, No. 7
0099-2240/08/$08.00+0     doi:10.1128/AEM.02601-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Solid-Phase Capture of Pathogenic Bacteria by Using Gangliosides and Detection with Real-Time PCR{triangledown} ,{dagger}

Prerak T. Desai, Marie K. Walsh, and Bart C. Weimer*

Department of Nutrition and Food Sciences, Western Dairy Center, Center for Microbe Detection and Physiology, Center for Integrated BioSystems, Utah State University, Logan, Utah 84322-4700

Received 17 November 2007/ Accepted 28 January 2008

We developed a method for concentrating pathogens from samples without enrichment. Immobilized gangliosides concentrated bacteria for detection with real-time PCR. A sensitivity of ~4 CFU/ml (3 h) in samples without competing microflora was achieved. Samples with competing microflora had a sensitivity of 40,000 CFU/ml. The variance was less than one cycle.

* Corresponding author. Mailing address: Utah State University, Center for Integrated BioSystems, 4700 Old Main Hill, Logan, UT 84322-4700. Phone: (435) 797-2753. Fax: (435) 797-0103. E-mail: bart.weimer{at}usu.edu

{triangledown} Published ahead of print on 8 February 2008.

{dagger} Approved as journal paper no. 7743 by the Utah Agricultural Experiment Station, Utah State University, Logan, UT 84322-4810.

Applied and Environmental Microbiology, April 2008, p. 2254-2258, Vol. 74, No. 7
0099-2240/08/$08.00+0     doi:10.1128/AEM.02601-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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