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Applied and Environmental Microbiology, April 2008, p. 2267-2274, Vol. 74, No. 8
0099-2240/08/$08.00+0     doi:10.1128/AEM.02381-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Solvent Stress Response of the Denitrifying Bacterium "Aromatoleum aromaticum" Strain EbN1{triangledown} ,{dagger}

Kathleen Trautwein,1,2,{ddagger} Simon Kühner,2,{ddagger} Lars Wöhlbrand,2 Thomas Halder,3 Kenny Kuchta,4 Alexander Steinbüchel,4 and Ralf Rabus1,2*

Institute of Chemistry and Biology of the Marine Environment (ICBM), University of Oldenburg, Carl-von-Ossietzky Str. 9-11, D-26111 Oldenburg, Germany,1 Max Planck Institute for Marine Microbiology, Celsiusstr. 1, D-28359 Bremen, Germany,2 TopLab GmbH, Fraunhoferstr. 18a, D-82152 Martinsried, Germany,3 Institute for Molecular Microbiology and Biotechnology, Westfälische Wilhelms University of Münster, Corrensstr. 3, D-48149 Münster, Germany4

Received 23 October 2007/ Accepted 28 January 2008

The denitrifying betaproteobacterium "Aromatoleum aromaticum" strain EbN1 degrades several aromatic compounds, including ethylbenzene, toluene, p-cresol, and phenol, under anoxic conditions. The hydrophobicity of these aromatic solvents determines their toxic properties. Here, we investigated the response of strain EbN1 to aromatic substrates at semi-inhibitory (about 50% growth inhibition) concentrations under two different conditions: first, during anaerobic growth with ethylbenzene (0.32 mM) or toluene (0.74 mM); and second, when anaerobic succinate-utilizing cultures were shocked with ethylbenzene (0.5 mM), toluene (1.2 mM), p-cresol (3.0 mM), and phenol (6.5 mM) as single stressors or as a mixture (total solvent concentration, 2.7 mM). Under all tested conditions impaired growth was paralleled by decelerated nitrate-nitrite consumption. Additionally, alkylbenzene-utilizing cultures accumulated poly(3-hydroxybutyrate) (PHB) up to 10% of the cell dry weight. These physiological responses were also reflected on the proteomic level (as determined by two-dimensional difference gel electrophoresis), e.g., up-regulation of PHB granule-associated phasins, cytochrome cd1 nitrite reductase of denitrification, and several proteins involved in oxidative (e.g., SodB) and general (e.g., ClpB) stress responses.

* Corresponding author. Mailing address: Institute of Chemistry and Biology of the Marine Environment (ICBM), University of Oldenburg, Carl-von-Ossietzky Str. 9-11, D-26111 Oldenburg, Germany. Phone: 49-441-798-3884. Fax: 49-441-798-3404. E-mail: rabus{at}icbm.de

{triangledown} Published ahead of print on 8 February 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} These authors contributed equally to this work.

Applied and Environmental Microbiology, April 2008, p. 2267-2274, Vol. 74, No. 8
0099-2240/08/$08.00+0     doi:10.1128/AEM.02381-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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  • Wohlbrand, L., Wilkes, H., Halder, T., Rabus, R. (2008). Anaerobic Degradation of p-Ethylphenol by "Aromatoleum aromaticum" Strain EbN1: Pathway, Regulation, and Involved Proteins. J. Bacteriol. 190: 5699-5709 [Abstract] [Full Text]  


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