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Applied and Environmental Microbiology, April 2008, p. 2526-2528, Vol. 74, No. 8
0099-2240/08/$08.00+0     doi:10.1128/AEM.02253-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

1-Aminocyclopropane-1-Carboxylate Deaminase Enhances Agrobacterium tumefaciens-Mediated Gene Transfer into Plant Cells{triangledown}

Satoko Nonaka,1 Masayuki Sugawara,2 Kiwamu Minamisawa,2 Ken-ichi Yuhashi,1 and Hiroshi Ezura1*

Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba 305-8572, Japan,1 Graduate School of Life Sciences, Tohoku University, Katahira, Aoba-ku, Sendai, Japan2

Received 3 October 2007/ Accepted 20 February 2008

Agrobacterium-mediated gene transfer is widely used for plant molecular genetics, and efficient techniques are required. Recent studies show that ethylene inhibits the gene transfer. To suppress ethylene evolution, we introduced 1-aminocyclopropane-1-carboxylate (ACC) deaminase into Agrobacterium tumefaciens. The ACC deaminase enhanced A. tumefaciens-mediated gene transfer into plants.

* Corresponding author. Mailing address: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba 305-8572, Japan. Phone and fax: 81-29-853-773. E-mail: ezura{at}gene.tsukuba.ac.jp

{triangledown} Published ahead of print on 29 February 2008.

Applied and Environmental Microbiology, April 2008, p. 2526-2528, Vol. 74, No. 8
0099-2240/08/$08.00+0     doi:10.1128/AEM.02253-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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