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Applied and Environmental Microbiology, April 2008, p. 2529-2533, Vol. 74, No. 8
0099-2240/08/$08.00+0     doi:10.1128/AEM.00975-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Species-Specific Identification of Campylobacters by PCR-Restriction Fragment Length Polymorphism and PCR Targeting of the Gyrase B Gene{triangledown} ,{dagger}

Susumu Kawasaki,1* Pina M. Fratamico,2 Irene V. Wesley,3 and Shinichi Kawamoto1

National Food Research Institute, Food Hygiene Team, 2-1-12 Kannondai, Tsukuba 305-8642, Japan,1 United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, Pennsylvania 19038,2 United States Department of Agriculture, Agricultural Research Service, National Animal Disease Center, 2300 Dayton Roa, Ames, Iowa 500103

Received 1 May 2007/ Accepted 11 February 2008

PCR-restriction fragment length polymorphism (RFLP) analysis of a 960-bp fragment of the Campylobacter gyrB gene with either DdeI or XspI restriction enzymes generated unique digestion patterns for 12 different Campylobacter species. In addition, PCR assays using species-specific primer sets targeting gyrB were specific for the respective Campylobacter species. Therefore, PCR-RFLP analysis and species-specific PCR assays based on the gyrB gene provide valuable tools for rapid and unambiguous identification of the majority of Campylobacter species.

* Corresponding author. Mailing address: National Food Research Institute, Food Hygiene Team, 2-1-12 Kannondai, Tsukuba 305-8642, Japan. Phone: 81-29-838-8067. Fax: 81-29-838-7996. E-mail: skawasa{at}affrc.go.jp

{triangledown} Published ahead of print on 22 February 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, April 2008, p. 2529-2533, Vol. 74, No. 8
0099-2240/08/$08.00+0     doi:10.1128/AEM.00975-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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