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Applied and Environmental Microbiology, April 2008, p. 2537-2539, Vol. 74, No. 8
0099-2240/08/$08.00+0     doi:10.1128/AEM.02288-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Removal of Free Extracellular DNA from Environmental Samples by Ethidium Monoazide and Propidium Monoazide{triangledown}

Andreas O. Wagner,* Cornelia Malin, Brigitte A. Knapp, and Paul Illmer

University of Innsbruck, Institute of Microbiology, Technikerstrasse 25, A-6020 Innsbruck, Austria

Received 8 October 2007/ Accepted 13 February 2008

Recently, new DNA extraction techniques (using ethidium monoazide and propidium monoazide) have been developed to discriminate between alive and dead bacterial cells. Nevertheless, for complex environmental samples, no data are available yet. In the present study, these new methods were applied to anaerobic-fermentor sludge and the results were compared to a conventional microbiological approach.

* Corresponding author. Mailing address: Institute of Microbiology, University of Innsbruck, Technikerstr. 25, A-6020 Innsbruck, Austria. Phone: 43 512 507 6006. Fax: 43 512 507 2928. E-mail: Andreas.Wagner{at}uibk.ac.at

{triangledown} Published ahead of print on 22 February 2008.

Applied and Environmental Microbiology, April 2008, p. 2537-2539, Vol. 74, No. 8
0099-2240/08/$08.00+0     doi:10.1128/AEM.02288-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bae, S., Wuertz, S. (2009). Discrimination of Viable and Dead Fecal Bacteroidales Bacteria by Quantitative PCR with Propidium Monoazide. Appl. Environ. Microbiol. 75: 2940-2944 [Abstract] [Full Text]  


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