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Applied and Environmental Microbiology, April 2008, p. 2550-2553, Vol. 74, No. 8
0099-2240/08/$08.00+0     doi:10.1128/AEM.02801-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Development of a Novel Screening Method for the Isolation of "Cronobacter" spp. (Enterobacter sakazakii){triangledown}

Carol Iversen,1,2 Patrick Druggan,3 Sandra Schumacher,1 Angelika Lehner,1 Claudia Feer,4 Karl Gschwend,4 Han Joosten,2 and Roger Stephan1*

Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, Switzerland,1 Quality and Safety Department, Nestlé Research Centre, Vers-chez-les-Blanc, CH-1000 Lausanne, Switzerland,2 Oxoid Ltd., Thermo Fisher Scientific, Basingstoke, Hampshire RG24 8PW, United Kingdom,3 QA and Food Safety Department, Hochdorf Nutritec AG, CH-6280 Hochdorf, Switzerland4

Received 12 December 2007/ Accepted 22 February 2008

A differential medium, "Cronobacter" screening broth, has been designed to complement agars based on hydrolysis of chromogenic {alpha}-glucopyranoside substrates. The broth was evaluated using 329 Enterobacteriaceae strains (229 target isolates), spiked/naturally contaminated samples, and a parallel comparison with current methods for raw materials, line/end products, and factory environment samples.

* Corresponding author. Mailing address: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Winterthurerstrasse 272, 8057 Zurich, Switzerland. Phone: 41-44-635-8651. Fax: 41-44-635-8908. E-mail: stephanr{at}fsafety.uzh.ch

{triangledown} Published ahead of print on 29 February 2008.

Applied and Environmental Microbiology, April 2008, p. 2550-2553, Vol. 74, No. 8
0099-2240/08/$08.00+0     doi:10.1128/AEM.02801-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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