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Applied and Environmental Microbiology, May 2008, p. 2894-2901, Vol. 74, No. 9
0099-2240/08/$08.00+0     doi:10.1128/AEM.00070-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Dynamics of the Methanogenic Archaeal Community during Plant Residue Decomposition in an Anoxic Rice Field Soil {triangledown}

Jingjing Peng, Zhe Lü, Junpeng Rui, and Yahai Lu*

College of Resources and Environmental Sciences, China Agricultural University, Beijing 100094, China

Received 9 January 2008/ Accepted 3 March 2008

Incorporation of plant residues strongly enhances the methane production and emission from flooded rice fields. Temperature and residue type are important factors that regulate residue decomposition and CH4 production. However, the response of the methanogenic archaeal community to these factors in rice field soil is not well understood. In the present experiment, the structure of the archaeal community was determined during the decomposition of rice root and straw residues in anoxic rice field soil incubated at three temperatures (15°C, 30°C, and 45°C). More CH4 was produced in the straw treatment than root treatment. Increasing the temperature from 15°C to 45°C enhanced CH4 production. Terminal restriction fragment length polymorphism analyses in combination with cloning and sequencing of 16S rRNA genes showed that Methanosarcinaceae developed early in the incubations, whereas Methanosaetaceae became more abundant in the later stages. Methanosarcinaceae and Methanosaetaceae seemed to be better adapted at 15°C and 30°C, respectively, while the thermophilic Methanobacteriales and rice cluster I methanogens were significantly enhanced at 45°C. Straw residues promoted the growth of Methanosarcinaceae, whereas the root residues favored Methanosaetaceae. In conclusion, our study revealed a highly dynamic structure of the methanogenic archaeal community during plant residue decomposition. The in situ concentration of acetate (and possibly of H2) seems to be the key factor that regulates the shift of methanogenic community.

* Corresponding author. Mailing address: College of Resources and Environmental Sciences, China Agricultural University, Beijing 100094, China. Phone and fax: 86 10 62733617. E-mail: yhlu{at}cau.edu.cn

{triangledown} Published ahead of print on 14 March 2008.

Applied and Environmental Microbiology, May 2008, p. 2894-2901, Vol. 74, No. 9
0099-2240/08/$08.00+0     doi:10.1128/AEM.00070-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rui, J., Peng, J., Lu, Y. (2009). Succession of Bacterial Populations during Plant Residue Decomposition in Rice Field Soil. Appl. Environ. Microbiol. 75: 4879-4886 [Abstract] [Full Text]  


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