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Applied and Environmental Microbiology, May 2008, p. 2921-2923, Vol. 74, No. 9
0099-2240/08/$08.00+0     doi:10.1128/AEM.00075-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Functional nodFE Genes Are Present in Sinorhizobium sp. Strain MUS10, a Symbiont of the Tropical Legume Sesbania rostrata{triangledown}

Hari B. Krishnan1,2* and Demosthenis Chronis2

Plant Genetics Research Unit, U.S. Department of Agriculture—Agricultural Research Service,1 Department of Plant Sciences, University of Missouri, Columbia, Missouri 652112

Received 10 January 2008/ Accepted 28 February 2008

We have cloned the nodFE operon from Sinorhizobium sp. strain MUS10. MUS10 NodF shows sequence homology to acyl carrier protein and enables an S. meliloti nodF mutant to effectively nodulate alfalfa. Our results demonstrate the occurrence of nodFE in a symbiont that nodulates a legume host not belonging to the galegoid group.

* Corresponding author. Mailing address: Plant Genetics Research Unit, USDA-ARS, 108 Curtis Hall, University of Missouri, Columbia, MO 65211. Phone: (573) 882-8151. Fax: (573) 884-7850. E-mail: KrishnanH{at}missouri.edu

{triangledown} Published ahead of print on 7 March 2008.

Applied and Environmental Microbiology, May 2008, p. 2921-2923, Vol. 74, No. 9
0099-2240/08/$08.00+0     doi:10.1128/AEM.00075-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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