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Applied and Environmental Microbiology, January 2009, p. 252-256, Vol. 75, No. 1
0099-2240/09/$08.00+0     doi:10.1128/AEM.01630-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Selective Extraction of Bacterial DNA from the Surfaces of Macroalgae{triangledown}

Catherine Burke, Staffan Kjelleberg,* and Torsten Thomas

School of Biotechnology and Biomolecular Sciences and Centre for Marine Bio-Innovation, The University of New South Wales, Sydney 2052, Australia

Received 15 July 2008/ Accepted 27 October 2008

A novel method has been developed for the selective extraction of DNA from surface-associated bacterial communities from the two model marine benthic algae Ulva australis and Delisea pulchra. The extracted DNA had no detectable contamination with host DNA, was recovered in high yield and quality, and was representative of the bacterial community on the algal surfaces. The DNA is suitable for a variety of subsequent applications, including the construction of large-insert clone libraries and metagenomic sequencing.

* Corresponding author. Mailing address: School of Biotechnology and Biomolecular Sciences and Centre for Marine Bio-Innovation, The University of New South Wales, Sydney 2052, Australia. Phone: (61 2) 93852102. Fax: (61 2) 93851779. E-mail: S.Kjelleberg{at}unsw.edu.au

{triangledown} Published ahead of print on 31 October 2008.

Applied and Environmental Microbiology, January 2009, p. 252-256, Vol. 75, No. 1
0099-2240/09/$08.00+0     doi:10.1128/AEM.01630-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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