This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Berger, E.
Right arrow Articles by Louw, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Berger, E.
Right arrow Articles by Louw, M.
Agricola
Right arrow Articles by Berger, E.
Right arrow Articles by Louw, M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2009, p. 271-274, Vol. 75, No. 1
0099-2240/09/$08.00+0     doi:10.1128/AEM.02430-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Impact of Inactivated Extracellular Proteases on the Modified Flagellin Type III Secretion Pathway of Bacillus halodurans{triangledown}

Eldie Berger, Erika du Plessis, Isak Gerber, Michael Crampton, Nolwandle Nxumalo, and Maureen Louw*

CSIR Biosciences, Box 395, Pretoria 0001, South Africa

Received 23 October 2008/ Accepted 28 October 2008

The flagellin type III secretion pathway of Bacillus halodurans BhFC01 ({Delta}hag) was modified by the inactivation of fliD. An in-frame flagellin gene fusion polypeptide construct was expressed, and the heterologous peptides were secreted as flagellin fusion monomers. The stability of the secreted monomers was significantly enhanced through gene-targeted inactivation of extracellular proteases.

* Corresponding author. Mailing address: CSIR Biosciences, Box 395, Pretoria 0001, South Africa. Phone: 27 12 841-2167. Fax: 27 12 841-3651. E-mail: melouw{at}csir.co.za

{triangledown} Published ahead of print on 7 November 2008.

Applied and Environmental Microbiology, January 2009, p. 271-274, Vol. 75, No. 1
0099-2240/09/$08.00+0     doi:10.1128/AEM.02430-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»