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Applied and Environmental Microbiology, May 2009, p. 3034-3038, Vol. 75, No. 10
0099-2240/09/$08.00+0     doi:10.1128/AEM.01862-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Inactivation of the SauI Type I Restriction-Modification System Is Not Sufficient To Generate Staphylococcus aureus Strains Capable of Efficiently Accepting Foreign DNA{triangledown}

Helena Veiga and Mariana G. Pinho*

Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Química e Biológica (ITQB), Universidade Nova de Lisboa (UNL), Oeiras, Portugal

Received 4 August 2008/ Accepted 11 March 2009

Genetic manipulation of Staphylococcus aureus is limited by the availability of only a single strain, RN4220, that is capable of easily accepting foreign DNA. Inactivation of the hsdR gene of the SauI type I restriction-modification system was shown previously to be responsible for the high transformation efficiency of RN4220 (D. E. Waldron and J. A. Lindsay, J Bacteriol. 188:5578-5585, 2006). However, deletion of this gene in three different S. aureus strains was not sufficient to make them readily transformable, which would be remarkably useful for genetic studies of this pathogenic organism. These results indicate that another unknown factor(s) is required for the transformable phenotype in S. aureus.

* Corresponding author. Mailing address: Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Química e Biológica (ITQB), Av. da Republica, 2781-901 Oeiras, Portugal. Phone: 351 21 4469544. Fax: 351 21 4411277. E-mail: mgpinho{at}itqb.unl.pt

{triangledown} Published ahead of print on 20 March 2009.

Applied and Environmental Microbiology, May 2009, p. 3034-3038, Vol. 75, No. 10
0099-2240/09/$08.00+0     doi:10.1128/AEM.01862-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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