This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dupont, C.
Right arrow Articles by Augustin, J.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dupont, C.
Right arrow Articles by Augustin, J.-C.
Agricola
Right arrow Articles by Dupont, C.
Right arrow Articles by Augustin, J.-C.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2009, p. 3069-3076, Vol. 75, No. 10
0099-2240/09/$08.00+0     doi:10.1128/AEM.02864-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Influence of Stress on Single-Cell Lag Time and Growth Probability for Listeria monocytogenes in Half Fraser Broth{triangledown}

Claire Dupont1,2* and Jean-Christophe Augustin1

Ecole Nationale Vétérinaire d'Alfort, Unité Microbiologie des Aliments—Sécurité et Qualité, 7 Avenue du Général de Gaulle, F-94704 Maisons-Alfort Cedex, France,1 AES Chemunex, Ker Lann—CS 17219, Rue Maryse Bastié, F-35172 Bruz Cedex, France2

Received 17 December 2008/ Accepted 14 March 2009

The impacts of 12 common food industry stresses on the single-cell growth probability and single-cell lag time distribution of Listeria monocytogenes were determined in half Fraser broth, the primary enrichment broth of the International Organization for Standardization detection method. First, it was determined that the ability of a cell to multiply in half Fraser broth is conditioned by its history (the probability for a cell to multiply can be decreased to 0.05), meaning that, depending on the stress in question, the risk of false-negative samples can be very high. Second, it was established that when cells are injured, the single-cell lag times increase in mean and in variability and that this increase represents a true risk of not reaching the detection threshold of the method in the enrichment broth. No relationship was observed between the impact on single-cell lag times and that on growth probabilities. These results emphasize the importance of taking into account the physiological state of the cells when evaluating the performance of methods to detect pathogens in food.

* Corresponding author. Mailing address: Unité Microbiologie des Aliments—Sécurité et Qualité, 7 avenue du Général de Gaulle, F-94704 Maisons-Alfort Cedex, France. Phone: 33 1 43967178. Fax: 33 1 43967121. E-mail: cdupont{at}vet-alfort.fr

{triangledown} Published ahead of print on 20 March 2009.

Applied and Environmental Microbiology, May 2009, p. 3069-3076, Vol. 75, No. 10
0099-2240/09/$08.00+0     doi:10.1128/AEM.02864-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»