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Applied and Environmental Microbiology, May 2009, p. 3153-3160, Vol. 75, No. 10
0099-2240/09/$08.00+0     doi:10.1128/AEM.00172-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Bile-Inducible Efflux Transporter from Bifidobacterium longum NCC2705, Conferring Bile Resistance{triangledown}

Miguel Gueimonde,1 Christel Garrigues,2 Douwe van Sinderen,3 Clara G. de los Reyes-Gavilán,1 and Abelardo Margolles1*

Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA), Consejo Superior de Investigaciones Científicas (CSIC), Villaviciosa, Asturias, Spain,1 Department of Physiology, Cultures and Enzymes Division, Chr. Hansen A/S, Hørsholm, Denmark,2 Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland3

Received 25 January 2009/ Accepted 10 March 2009

Bifidobacteria are normal inhabitants of the human gut. Some strains of this genus are considered health promoting or probiotic, being included in numerous food products. In order to exert their health benefits, these bacteria must overcome biological barriers, including bile salts, to colonize and survive in specific parts of the intestinal tract. The role of multidrug resistance (MDR) transporters in bile resistance of probiotic bacteria and the effect of bile on probiotic gene expression are not fully understood. In the present study, the effect of subinhibitory concentrations of bile on the expression levels of predicted MDR genes from three different bifidobacterial strains, belonging to Bifidobacterium longum subsp. longum, Bifidobacterium breve, and Bifidobacterium animalis subsp. lactis, was tested. In this way, two putative MDR genes whose expression was induced by bile, BL0920 from B. longum and its homolog, Bbr0838, from B. breve, were identified. The expression of the BL0920 gene in Escherichia coli was shown to confer resistance to bile, likely to be mediated by active efflux from the cells. To the best of our knowledge, this represents the first identified bifidobacterial bile efflux pump whose expression is induced by bile.

* Corresponding author. Mailing address: Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA), Consejo Superior de Investigaciones Científicas (CSIC), Ctra. Infiesto s/n, 33300 Villaviciosa, Asturias, Spain. Phone: 34 985892131. Fax: 34 985892233. E-mail: amargolles{at}ipla.csic.es

{triangledown} Published ahead of print on 20 March 2009.

Applied and Environmental Microbiology, May 2009, p. 3153-3160, Vol. 75, No. 10
0099-2240/09/$08.00+0     doi:10.1128/AEM.00172-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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