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Applied and Environmental Microbiology, May 2009, p. 3323-3330, Vol. 75, No. 10
0099-2240/09/$08.00+0     doi:10.1128/AEM.02399-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Suppression of the Bacterial Spot Pathogen Xanthomonas euvesicatoria on Tomato Leaves by an Attenuated Mutant of Xanthomonas perforans{triangledown}

A. P. Hert,1 M. Marutani,1 M. T. Momol,2 P. D. Roberts,3 S. M. Olson,2 and J. B. Jones1*

Department of Plant Pathology, University of Florida, Gainesville, Florida 32611,1 North Florida Research and Education Center, University of Florida, Quincy, Florida 32060,2 Southwest Florida Research and Education Center, University of Florida, Immokalee, Florida 341423

Received 19 October 2008/ Accepted 9 March 2009

A bacteriocin-producing strain of the bacterial spot of tomato plant pathogen, Xanthomonas perforans, with attenuated pathogenicity was deployed for biocontrol of a bacteriocin-sensitive strain of the genetically closely related bacterial spot of tomato plant pathogen, X. euvesicatoria. The attenuated mutant (91-118{Delta}opgH{Delta}bcnB) of X. perforans was tested in leaf tissue and shown to significantly inhibit internal populations of the wild-type X. euvesicatoria strain although significantly less than the wild-type 91-118 strain, whereas in a phyllosphere inhibition assay, the mutant strain reduced epiphytic populations comparably to 91-118. Thus, the attenuated mutant limited the sensitive bacterium more efficiently on the leaf surface than inside the leaf. In field experiments, weekly application of 91-118{Delta}opgH{Delta}bcnB significantly reduced X. euvesicatoria populations compared to the growers’ standard control (copper hydroxide and mancozeb applied weekly and acibenzolar-S-methyl applied every 2 weeks). The biological control agent, 91-118{Delta}opgH{Delta}bcnB, applied every 2 weeks also significantly reduced X. euvesicatoria populations in one season but was not significantly different from the growers’ standard control. Potentially, attenuated pathogenic strains could be deployed as biological control agents in order to improve disease control of foliar plant pathogens.

* Corresponding author. Mailing address: Department of Plant Pathology, University of Florida, P.O. Box 110680, Gainesville, FL 32611. Phone: (352) 392-3631. Fax: (352) 392-6532. E-mail: JBJones{at}ufl.edu

{triangledown} Published ahead of print on 13 March 2009.

Applied and Environmental Microbiology, May 2009, p. 3323-3330, Vol. 75, No. 10
0099-2240/09/$08.00+0     doi:10.1128/AEM.02399-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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