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Applied and Environmental Microbiology, May 2009, p. 3352-3354, Vol. 75, No. 10
0099-2240/09/$08.00+0 doi:10.1128/AEM.02407-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

School of Biotechnology and Biomolecular Sciences, University of New South Wales, Randwick, New South Wales 2052, Australia,1 Department of Biological Sciences, Macquarie University, North Ryde, New South Wales 2019, Australia2
Received 21 October 2008/ Accepted 10 March 2009
Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.
Published ahead of print on 20 March 2009.
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